Effect of gamma radiation on adult mice ovary: A model for premature ovarian failure due to loss of early germinal cells
Introduction: In this study, we report an animal model wherein there is loss of ovarian follicular reserve due to irradiation.
Materials and Methods: Adult female mice of 3 months age were exposed to gamma radiation (total dose of 6 Gy). Seven days after the exposure, the animals were sacrificed, ovaries were collected and biochemical and histological observations were conducted.
Results: The ovaries of animals exposed to radiation showed a significant increase (P < 0.05) in the concentration of reactive oxygen species and activities of catalase (CAt) and superoxide dismutase (SOD)concomitant with a drastic reduction (P < 0.02) in the number of primordial (84% loss) and primary follicles (92% loss) in the ovary and irregular estrous cycle. The number of pre-antral and antral follicles, malondialdehyde (MDA) concentration, ferric reducing anti-oxidant power (FRAP) and the gel electrophoretic profile of ovarian DNA did not show significant variation.
Conclusion: The results revealed that at the given dose level, radiation exposure causes loss of follicular reserve and abnormal estrous cyclicity, without causing DNA breaks or increase in lipid peroxidation. Our findings confirmed that, this protocol is suitable for studying different aspects of premature ovarian failure in adults.
Keywords: Gamma radiation, Oxidative stress, Ovarian failure, Cancer
Please cite this paper as: Saranya MK, Athira VR, Shivanandappa T, Yajurvedi HN. Effect of gamma radiation on adult mice ovary: A model for premature ovarian failure due to loss of early germinal cells. J Radiobiol 2017;4(1):e02.
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