The effect of whole body gamma irradiation on nitric oxide pathway of rat’s aorta
Introduction: Studies show that ionizing radiation impairs the so called blood vessels relaxation through inhibition of iNOS transcription without causing any morphological changes in them. This effect has been proven in Low Dose and Whole body irradiation. Thus this study focuses on the effect of chronic whole body irradiation on susceptibility of blood vessels to nitric oxide.
Methods: Twenty five adult male Wistar rats (weight 200 to 250 g) were divided into 5 groups, each allocated to chronic 6 days whole body cumulative doses of 0.84, 1, 1.5, 2, 2.5 gray using 137Cs as gamma ray source (dose rate of 0.014574 Gy/min). experiments performed shortly after 6 days irradiation by anaesthetizing rats using intraperitoneal injection of sodium pentobarbital (30 mg/kg) and excising thoracic aorta and immediately placing them in 4 ºC Krebs’ bicarbonate buffer. Each aortic ring was cut into 2-3 mm segments free from adherent connective tissue. The rings were suspended horizontally between two stirrups in organ chambers filled with 25 mL Krebs’ solution at 37 ºC and aerated continuously with 95% O2 and 5% CO2. One stirrup was connected to an anchor and the other was connected to a force transducer for recording isometric tension the mechanical responses of the segments. The data from 1st, 5th, 10th and 15th minutes was analyzed using ANOVA and Duncan at p≤ 0.05 as significance level. Results were expressed as Mean ± SD.
Results: Results showed no significance differences between responses of isolated aorta strips.
Conclusions: It can be concluded that exposure of chronic whole body radiation may not influence the susceptibility of blood vessels to NO.
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