Effect of extremely low-frequency (50 Hz) field on proliferation rate of human adipose-derived mesenchymal stem cells

Daryoush Shahbazi-Gahrouei, Shahnaz Razavi, Marzieh Salimi


Introduction: The effects of non-ionizing extremely low-frequency magnetic fields (ELF-EMF) chronic exposure on human beings due to its potential health hazards has become a focus of interest since many years ago. Adipose tissue has been known as a source of multipotent stromal stem cells (MSCs), which can be obtained by a less invasive method and in large amounts compared with bone marrow derived stem cells (BMSCs). This study aimed to consider the effect of ELF-EMF on proliferation and differentiation rates of adipose-derived stem cells (ADSCs).
Methods: The effect of ELF-EMF with intensity of 0.5 and 1 mT and 50 Hz on proliferation rates of hADSCs at 20 and 40 min/day for 7 days was assessed. MTT assay was used to determine the growth and metabolism of cells and Trypan blue test was also done for cell viability.
Results: The lively hMSCs counting after ELF-EMF exposure at 7th day by Trypan blue staining showed that the proliferation in all groups of exposure was higher than the sham groups. There was an inconsistence result of MTT assay Trypan blue in group of 1 mT and 40 min/day exposure. In contrast to results of MTT assay, the proliferation rate of hMSCs in this group assessed by Trypan blue staining was significantly more than the sham group (P<0.05)
Conclusion: The results showed that 0.5 and 1 mT magnetic field strengths can be used to the proliferation rates of the (human mesenchymal stem cells) hMSCs derived from adipose tissue regarding the duration of exposure, although the effective mechanisms in this process are still unknown.

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